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If an Invitrogen™ antibody isn't going to carry out as explained on our Web-site or datasheet,we are going to replace the item for free of charge to you, or offer you a credit rating for the long run invest in.*

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene has long been noticed in many tumors, which results in chromosome instability, and so may well lead to tumorigenesis. This protein was uncovered to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a critical purpose in selling cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are already described. Two additional splice variants ended up noted but specific nucleotide sequence information and facts just isn't yet readily available.

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Blocking peptides are peptides that bind particularly on the target antibody and block antibody binding. These peptide typically incorporates the epitope acknowledged by the antibody.

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This guideline outlines even further attainable optimization of cellular and immunoassay parameters to ensure the very best benefits are...

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This information outlines further doable optimization of cellular and immunoassay parameters to be certain the very best outcomes are...

Antibodies certain to the blocking peptide now not bind towards the epitope within the concentrate on protein. By comparing the staining from the blocked antibody versus the antibody by yourself, you can see which staining is particular.

Goal Information and facts Cyclin E belongs on the extremely conserved cyclin household, whose users are characterised by a extraordinary periodicity in protein abundance with the mobile cycle. Cyclins purpose as regulators of CDK kinases. Distinct cyclins show unique expression and degradation designs which lead to your temporal coordination of every mitotic function. Cyclin E kinds a fancy with and capabilities as a regulatory subunit of CDK2, whose exercise is required for cell cycle G1/S changeover. This protein accumulates for the G1-S period boundary and is also degraded as cells development by S section.

The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both equally antibodies...

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Clicking the photographs or links will redirect you check here to a website hosted by BenchSci that provides third-occasion scientific written content. Neither the content material nor the BenchSci technological innovation and procedures for collection have been evaluated by us; we are supplying them as-is and without having warranty of any sort, like to be used or software on the Thermo Fisher Scientific solutions introduced.